WebJul 14, 2024 · Our optical tweezers setup is based on an Olympus IX73 inverted microscope, as shown in Figure 1A. A 1064-nm laser (Amonics, Hong Kong, AFL-1064-37-R-CL, cw) was used as the trapping laser source. The laser beam was expanded to fulfill the pupil of objective (8 mm) with the beam expander. WebBasic Theory of Optical Tweezers. Optical Tweezers, or traps as they are often called, are created by using a high numerical aperture objective to tightly focus a laser beam, thereby creating a spot where a particle with dimensions on the order of microns will experience a force due to transfer of momentum from the scattering of photons.
Exploring mechanochemical processes in the cell with optical tweezers …
WebOptical tweezers have emerged as a powerful tool for the non-invasive trapping and manipulation of colloidal particles and biological cells 1,2 . However, the diffraction limit precludes the low-power trapping of nanometre-scale objects. Substantially increasing the laser power can provide enough trapping potential depth to trap nanoscale objects. WebExperimental Set-up Our optical tweezers set-up consists of: a source laser, optical components to guide and refocus the beam, a sample slide with translational stages, a white light source, and a CCD camera and computer for acquiring data. The set-up can be seen in figure 2 at the bottom of the page. A 637nm, 70mW laser is guided through a fiber cv. jati utama
(PDF) Optofluidic Tweezers: Manipulation of Oil Droplets with 10 5 ...
WebNov 11, 2024 · Right: typical set-up and technology used in a neutral-atom optical tweezer trapping experiment. A spatial light modulator (SLM) and set of acousto-optic deflectors (AODs) are used to create ... WebMost frequently, optical tweezers are built by modifying a standard optical microscope. These instruments have evolved from simple tools to manipulate micron-sized objects to sophisticated devices under computer control that can measure displacements and … انتخاب عدد رندوم در پایتون