Immobilized template assays
Witryna15 sty 2000 · All of the immobilized DNA templates containing appropriate regulatory regions were fully competent for transcription and translation reactions and several could be re-used in serial reactions. ... Assays utilizing post-PCR immobilized DNA have been developed for a myriad of applications, including: the detection of single or multiple ... Witryna10 cze 2008 · The immobilized template assays were performed as shown in Fig. 1C; however, the nuclear extract was preincubated with Lys-CoA or curcumin, selective inhibitors of CBP/p300 acetyltransferase activity (15, 16). Each inhibitor blocks the acetyltransferase activity of CBP/p300 by a distinct mechanism.
Immobilized template assays
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Witryna2 maj 2013 · In this paper, we describe a peptide library designed by computational modelling and the selection of two peptide sequences showing affinity towards the mycotoxin, ochratoxin A (OTA). A virtual library of 20 natural amino acids was used as building blocks to design a short peptide library against ochratoxin A template using … Witryna1 sie 2002 · For immobilized-template assays, an EcoRI-NdeI fragment (ca. 600 bp) from plasmid pA4xMLΔ53 (29, 30), which contains the HNF-4 cognate sites and the core promoter elements, was filled in with biotinylated dATP by using the Klenow fragment of DNA Pol I, gel purified, and bound to M280-streptavidin Dynabeads (Dynal), as …
WitrynaImmobilized template assay for Mot1 1. Anneal oligos in 100 µl of 1X TE+ 0.1M NaCl by heating at 100°C for 5 mins and then slow cooling overnight. Add 10µg of … Witryna13 lis 2024 · Indeed, MEF2B binding to essential enhancer DNA in EMSA and immobilized template assays was dependent upon the presence of OCT2 and its co …
Witryna29 kwi 2011 · ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and … Witryna25 paź 2010 · To identify the specific histone targets of acetylation by p300, we used a chromatin-based immobilized template assay comprised of the model human T-cell …
Witryna7 kwi 2024 · Plasmonic nanoprobes consisting of gold nanoparticles (AuNPs) and biomolecules are used due to their LSPR properties. In 2024, Kim et al. proposed a sensitive sandwich assay to detect the surface antigen of the hepatitis B virus (HBsAg). They used a glass substrate fabricated with AuNPs, and the antibody was …
Witryna8 sty 2009 · Immobilized template assay. Biotinylated templates were prepared by PCR as described previously, with pSH515 as a template (Ranish et al, 1999). … portable media player iriverWitryna24 maj 2024 · Examples of such assays include polymerase-based amplifying protocols such as PCR and the Rolling Circle Amplification (RCA) method, which has been developed into a single molecule detection scheme by combining it with fluorescence labelling. ... (Vector Laboratories, Burlingame, CA, USA). Immobilized DNA … portable mediachance dynamic auto painterWitrynaimmobilized template assay to investigate activator and Ac-CoA-dependent disassembly of nucleosomes from a native promoter. We compared chromatin … portable medium duty shooting riserWitrynaBoth substances are banned by the World Anti-Doping Agency (WADA). The nanoMIPs similar to natural antibodies were used for the detection of atenolol and labetalol in competitive ELISA assay in water and human urine samples. The nanoMIPs were synthesized using methodology of solid-phase synthesis with immobilized template. irs approved efiling sitesWitrynaThe immobilized template recruitment assay can be used to circumvent this limitation. In this procedure, a biotinylated DNA fragment is immobilized on streptavidin-coated … irs approved e file providerWitryna6 paź 2024 · For the immobilized template assay, the same procedure was followed except for collecting the pellet and supernatant fractions at the desired time points. To map TOP2B, indicated restriction enzymes were added to the reactions at the appointed time points and allowed to sit for 15 min at 30°C. The pellet fraction was dissolved in … portable medical privacy screens ukWitryna25 paź 2010 · We utilized an in vitro immobilized template assay to investigate activator and Ac-CoA-dependent disassembly of nucleosomes from a native promoter. We compared chromatin templates composed of a mixture of wild-type histones and histones carrying lysine to arginine (K → R) N-terminal tail mutations. portable medication alarm timers