Cisplatin cytof

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August undefined, 2024

WebCisplatin is a platinum-based agent whose nephrotoxicity is thought related to the chloride in the cis position. Cisplatin gains entry into tubular cells via uptake by the OCT-2 … WebFor use with CyTOF 2, Helios, and CyTOF XT . Contents . About This Document ... Cell-ID Cisplatin—100 µL 201064 –20 ºC in single-use aliquots Cell-ID Cisplatin-194Pt—100 µL 201194 Cell-ID Cisplatin-195Pt—100 µL 201195 Cell-ID Cisplatin-196Pt—100 µL 201196

The Temperature-Dependent Effectiveness of Platinum-Based …

WebJan 21, 2024 · Cisplatin is commonly used as a viability stain in mass cytometry studies, however, recent work by Mei et al. has demonstrated that cisplatin can also be used to label antibodies, complicating the … WebJun 19, 2024 · Cisplatin, a chemotherapeutic agent, previously employed to specifically label dead cells for mass cytometry 4 contains a platinum atom which is found as six naturally occurring isotopes with 194... tsho la pass nepal

Laser ablation-ICP-TOFMS imaging of germ cell tumors of …

http://cytoforum.stanford.edu/viewtopic.php?f=3&t=374 Web• Cell-ID™ Cisplatin (Cat# 201064) • Cell-ID™ Intercalator-103Rh (DVS Sciences Cat# 201103A [500µM] or 201103B [2000µM]) ... Leave cells pelleted until ready to run on CyTOF®. Immediately prior to CyTOF data acquisition, adjust cell concentration to 2.5-5 x 10. 5 /ml with MaxPar Water and filter cells into cell strainer cap tubes. Weboperation he received cisplatin at a total dose of 100 mg m 2. A 13-year old male patient (patient 2) was diagnosed with a malignant secreting germ cell tumor. He received four cycles of chemotherapy, including cisplatin with a cumulative dose of 400 mg m 2 before resection of visible remaining parts of the tumor and subsequent radiotherapy. philton

Imaging Mass Cytometry - Chang - 2024 - Wiley Online Library

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WebMay 17, 2024 · We developed a 40+ parameter CyTOF panel and demonstrate that compared to flow cytometry, CyTOF yields analogous quantification of cell lineages … WebJul 25, 2024 · The first platinum anticancer drug cisplatin is widely used to treat various types of cancers including ovarian, testicular, bladder, colorectal, lung, and head and neck cancers [ 16, 17 ]. Cisplatin crosslinks with purine bases on the DNA, interfering with DNA repair mechanisms, causing DNA damage and inducing apoptosis [ 16 ]. tsholo monedihttp://cytoforum.stanford.edu/viewtopic.php?f=4&t=2197 tsholo cv

"WebCisplatin resistance in ovarian cancer remains a complex problem as tumors frequently develop resistance against drugs, a mechanism sometimes mediated by ATP-Binding Cassette transporters. Our goal was to find compounds restricting their inhibition " - Cisplatin cytof

Cisplatin cytof

Cisplatin (Intravenous Route) Side Effects - Mayo Clinic

WebDec 25, 2024 · Cisplatin treatment reduced tumor growth, number of lung metastasis, and the splenomegaly of 4T1 tumor bearing mice. We showed that cisplatin inhibited … WebSep 10, 2015 · Cisplatin-labeling of antibody increases the analytical capacity of the CyTOF ® platform by two channels based on …

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WebSimilarly, short treatment of cells with the platinum-based reagent cisplatin is used in CyTOF to distinguish live from dead cells (25) (Figure 1A). ... View in full-text Context 7 … WebApr 13, 2024 · CAR T cell therapy has revolutionized the treatment of leukaemia 1, lymphoma 2,3,4,5 and multiple myeloma 6, but has had limited success to date in glioblastoma and, more generally, in solid ...

WebCisplatin is ideally detected in the 195Pt channel of the CyTOF® system. PBMC were incubated for three days in media alone (left) or with PHA (right). Cells were then stained … WebFigure S7: Gating strategy for Mass cytometry: The gating strategy for CyTOF by mass cytometry is shown. i) Without light scatter to identify individual cells DNA content is used (rhodium and ...

WebWe support the scientific community in the quest to solve unanswered challenges in health and disease across basic, translational and clinical research, including the fields of cancer, inflammatory diseases, immunotherapies and environmental monitoring. Harnessing the power of proprietary CyTOF® and microfluidics technologies, Fluidigm products provide … WebJul 25, 2024 · The cellular uptake of platinum-based chemotherapeutic agents (cisplatin, oxaliplatin, and carboplatin) after hyperthermia treatment was assessed using cytometry …

WebNov 5, 2014 · The way that I typically incorporate cisplatin viability staining into my live cell staining protocols is to perform my antibody staining (in 100uL FACS buffer), then add 100uL of 2X cisplatin (diluted 1:500 from Fluidigm stock in PBS) and incubate for 5 minutes RT, then wash and fix. This reduces wash steps, which minimizes cell loss and seems ...

WebContribute to JinmiaoChenLab/cytofkit2 development by creating an account on GitHub. tsholiceWebCisplatin uptake and effects on proliferation BxPC-3 pancreatic cancer cells and ME-180 cervical cancer cells exposed to cisplatin in vitro were pulse-labeled with 10 mM IdU for 30 min and analyzed for platinum and iodine content by mass cytometry. Figure 1a illustrates single cell measurements taken from control and cisplatin-treated cells ... tsholo gumedeWebCisplatin-labeling of antibody increases the analytical capacity of the CyTOF(®) platform by two channels based on available reagents, and has the potential to add … tsholo peeters tsholham bootsWebJul 8, 2024 · Since Pt is an integral part of cisplatin and most cisplatin attaches to the Ab via free thiols (omitting reduction w TCEP strongly interferes with Ab labeling success), … tsholohelo primary schoolWebFeb 3, 2024 · Imaging Mass Cytometry (IMC) is an expansion of mass cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light … phil tone sapphireWebMay 31, 2024 · Conclusion: CyTOF is therefore a powerful and promising method to quantify cisplatin accumulation that may be used in the clinical setting to improve and personalize cancer treatment. Keywords: ABCC2 aurones cisplatin resistance cyTOF inhibitors mass cytometry multidrug resistance ovarian cancer philton alfred